龙牙百合两种病害致病菌的分离、鉴定及抑制效应研究

文章编号：  1000-3142（2018）01-0109-10

Isolation，identification and inhibiting effects of pathogens that caused two kinds of diseases in Lilium brownii var. viridulum

QIU Shuo， XIA Ke， LI Xiujuan， ZHOU Hao， TANG Fengluan， ZHAO Zhiguo， ZHAO Jian*

（ Guangxi Institute of Botany， Guangxi Zhuang Autonomous Region and  Chinese Academy of Sciences， Guilin 541006， Guangxi， China ）

Abstract：  The pathogens that caused two kinds of diseases in Lilium brownii var. viridulum were isolated using usual tissue isolation. Strains were cultivated， purified， reinoculated and reisolated. Morphological and molecular biological techniques were used to identify the strains. And three plant growth regulators were used to study the inhibiting effects on two pathogens. The results showed that two kinds of diseases were leaf blight （A） and penicilliosis （B） by the symptoms， and four strains and three strains were isolated from infected leaves， respectively. But only A-4 and B-2 caused two kinds of diseases， respectively. The incidences of A-4 and B-2 disease reinoculated in the field were all 100 %. A-4 is a kind of fungi belonging to the genus Botrytis， and its colonial morphology is a circular form， with colorless mycelium， prostrate on the medium， aerial， diaphragms and branches. There are conidium and acrospore. B-2 is a kind of fungi belonging to the genus Penicillium， and its colonial morphology is a circular form or irregular， with white mycelium outside the circle and turquoise in the central， the mycelium prostrate on the medium and diaphragms. There were broom conidium and spherical spores. At last， the sequence of internal transcribed spacer （ITS） region of A-4 and B-2 were analyzed， the length was 522 bp and 551 bp， respectively. The sequence were compared with other species in the Genbank， respectively. The symptom in the field indicates that the pathogen of Lilium brownii var. viridulum leaf blight（A） and penicilliosis are Botrytis elliptica and Penicillium expansum. The inhibiting effects of three plant growth regulators on two pathogens indicated that two pathogens could not be fully inhibited by 0.1-1.0 mmol·L-1 SA， but could be fully inhibited by 0.5-1.0 mmol·L-1BRs or Me-JA.

推荐访问:致病菌 两种 病害 百合 抑制

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